Found 1197 results, showing the newest relevant preprints. Sort by relevancy only.Update me on new preprints

Single-cell immuno-mechanics: rapid viscoelastic changes are a hallmark of early leukocyte activation.

Using an innovative micropipette rheometer, we show in three different cell types that when stimulated by microbeads mimicking target cells, leukocytes become up to ten times stiffer and more viscous. Expand abstract.
7 hours ago
8/10 relevant
bioRxiv

Visualization of viral infection dynamics in a unicellular eukaryote and quantification of viral production using VirusFISH

VirusFISH allowed the visualization and quantification of the fraction of infected cells during an infection experiment. Expand abstract.
9 hours ago
4/10 relevant
bioRxiv

Chronic viral infection promotes early germinal center exit of B cells and impaired antibody development.

To address this question, we used single-cell transcriptional profiling of virus-specific GC B cells to test the hypothesis that chronic viral infection disrupted GC B cell fate decisions leading to suboptimal humoral immunity. Expand abstract.
1 day ago
7/10 relevant
bioRxiv

Testes of DAZL null sheep lack spermatogonia and maintain normal somatic cells

Somatic cells within their testes were morphologically intact and expressed normal levels of somatic cell-specific marker genes, indicating that the germ cell niche remained intact. Expand abstract.
2 days ago
6/10 relevant
bioRxiv

Developmental trajectory of pre-hematopoietic stem cell formation fromendothelium

To examine the cell and molecular transitions during the EHT, and the heterogeneity of HSPCs within IACs, we profiled ~37,000 cells from the caudal arteries of embryonic day 9.5 (E9.5) to E11.5 mouse embryos by single-cell transcriptome and chromatin accessibility sequencing. Expand abstract.
2 days ago
4/10 relevant
bioRxiv

Semi-supervised identification of cell populations in single-cell ATAC-seq

In order to address this problem, we present Semi-Supervised Identification of Populations of cells in scATAC-seq data (SSIPs), a semi-supervised approach that integrates bulk and single-cell data through a generalizable network model featuring two types of nodes. Expand abstract.
3 days ago
10/10 relevant
bioRxiv

Tuning cell motility via cell tension with a mechanochemical cell migration model

These model predictions are consistent with our single-cell experiments and other experimental results. Expand abstract.
4 days ago
4/10 relevant
bioRxiv

Tempora: cell trajectory inference using time-series single-cell RNA sequencing data

However, when time series data is available, these methods do not consider the available time information when ordering cells and are instead designed to work only on a single scRNAseq data snapshot. Expand abstract.
4 days ago
10/10 relevant
bioRxiv

Single cell multiomic analysis of T cell exhaustion in vitro

In this study, we demonstrate the power of a single-cell multiomic approach to comprehensively characterize T cells and to precisely monitor changes in differentiation, activation and exhaustion signatures in response to different activation protocols. Expand abstract.
6 days ago
10/10 relevant
bioRxiv

Transcriptional reprogramming of distinct peripheral sensory neuron subtypes after axonal injury

By transcriptionally profiling sensory ganglia at single-cell resolution, we find that different somatosensory neuronal subtypes undergo a remarkably consistent and dramatic transcriptional response to peripheral nerve injury that both promotes axonal regeneration and suppresses cell identity. Expand abstract.
7 days ago
4/10 relevant
bioRxiv