Found 298 results, showing the newest relevant preprints. Sort by relevancy only.Update me on new preprints

The network structure and eco-evolutionary dynamics of CRISPR-induced immune diversification

Our findings emphasize the role that network structure plays in CRISPR-induced host-virus coevolution, providing one explanation for existing host/viral diversity in natural and empirical systems. Expand abstract.
7 hours ago
9/10 relevant
bioRxiv

Minimal genome-wide human CRISPR-Cas9 library

MinLibCas9 improves the efficiency of CRISPR-Cas9 loss-of-function screens and expands their application to complex models and assays. Expand abstract.
29 hours ago
9/10 relevant
bioRxiv

CRISPR-Cas12a/Cpf1-assisted precise, efficient and multiplexed genome-editing in Yarrowia lipolytica

To demonstrate the utility of CRISPR-Cpf1, we have optimized the CRISPR-Cpf1 system and achieved high-editing efficiency for two counter-selectable markers in the industrially-relevant oleaginous yeast Yarrowia lipolytica: arginine permease (93% for CAN1) and orotidine 5'-phosphate decarboxylase (~96% for URA3). Expand abstract.
2 days ago
9/10 relevant
bioRxiv

RNA-guided Retargeting of Sleeping Beauty Transposition in Human Cells

Our data indicate that the targeting mechanism specified by CRISPR/Cas9 forces integration into genomic regions that are otherwise poor targets for SB transposition. Expand abstract.
2 days ago
4/10 relevant
bioRxiv

TUTase mediated site-directed access to clickable chromatin employing CRISPR-dCas9

A terminal uridylyl transferase (TUTase) was repurposed to construct an sgRNA containing multiple minimally invasive bioorthogonal clickable handles, which served as a Trojan horse on CRISPR-dCas9 system to guide synthetic tags site-specifically on chromatin employing copper-catalyzed or strain-promoted click reactions. Expand abstract.
3 days ago
9/10 relevant
bioRxiv

SNP-CRISPR: a web tool for SNP-specific genome editing

Given these capabilities, SNP-CRISPR has a wide range of potential research applications in model systems and potential applications for design of sgRNAs for disease-associated mutant correction. Expand abstract.
3 days ago
9/10 relevant
bioRxiv

Spatiotemporal Control of CRISPR/Cas9 Function in Cells and Zebrafish using Light-Activated Guide RNA

Caging confers complete suppression of gRNA:target dsDNA hybridization and rapid restoration of CRISPR/Cas9 function upon optical activation. Expand abstract.
9 days ago
9/10 relevant
bioRxiv

CRISPR screening of porcine sgRNA library identified host factors essential for Japanese encephalitis virus replication

Thus, beyond our development of the largest CRISPR-based functional genomic screening platform for pig research to date, this work deepens our basic understanding of flavivirus infection and identifies multiple potentially vulnerable targets for the development of medical and breeding technologies to prevent and treat diseases caused... Expand abstract.
9 days ago
9/10 relevant
bioRxiv

Deep profiling reveals substantial heterogeneity of integration outcomes in CRISPR knock-in experiments

CRISPR/Cas technologies have transformed our ability to add functionality to the genome by knock-in of payload via homology-directed repair (HDR). Expand abstract.
10 days ago
9/10 relevant
bioRxiv

Defining Essential Enhancer for Pluripotent stem cells using Features Oriented CRISPR-Cas9 Screen

In this study, we performed a Features Oriented CRISPR Utilized Systematic (FOCUS) screen of OCT4-bound CREs using CRISPR/Cas9 to identify functional enhancers important for pluripotency maintenance in mouse ES cells. Expand abstract.
10 days ago
9/10 relevant
bioRxiv