Found 363 results, showing the newest relevant preprints. Sort by relevancy only.Update me on new preprints

Dietary supplementation with PUFAs rescues the eggshell defects caused by seipin mutations in C. elegans

Three full gene deletion mutants, generated by CRISPR/Cas9, displayed penetrant embryonic lethality. Expand abstract.
4 days ago
4/10 relevant
bioRxiv

Synthetic Apomixis an Old Enigma to Preserve Hybrid Vigor

In present review, we discussed the current development in plants by utilizing CRISPR/Cas9 technology and its possibility of promoting apomixis in crops to preserve hybrid vigour. Expand abstract.
11 days ago
6/10 relevant
Preprints.org

DCyFIR: a high-throughput CRISPR platform for multiplexed G protein-coupled receptor profiling and ligand discovery

A major advantage of DCyFIR is that GPCRs and other assay components are CRISPR-integrated directly into the yeast genome, making it possible to decode ligand specificity by profiling mixtures of GPCR-barcoded yeast strains in a single tube. Expand abstract.
11 days ago
9/10 relevant
bioRxiv

Fast and efficient generation of knock-in human organoids using homology-independent CRISPR/Cas9 precision genome editing

CRISPR-HOT simplifies genome editing in human organoids. Expand abstract.
12 days ago
9/10 relevant
bioRxiv

TOP the Transcription Orientation Pipeline and its use to investigate the transcription of non-coding regions: assessment with CRISPR direct repeats and intergenic sequences.

TOP was applied to all the available RNA-Seq Illumina sequencing archives from species possessing a CRISPR array, allowing comparisons with programs dedicated to the orientation of CRISPR repeats. Expand abstract.
12 days ago
9/10 relevant
bioRxiv

CRISPR-mediated activation of biosynthetic gene clusters for bioactive molecule discovery in filamentous fungi

This is the first demonstration of CRISPRa in filamentous fungi, providing a framework for CRISPR-mediated transcriptional activation of fungal BGCs. Expand abstract.
13 days ago
9/10 relevant
bioRxiv

Ares-GT: design of guide RNAs targeting multiple genes for CRISPR-Cas experiments

Motivation: There is a lack of tools to design guide RNAs for CRISPR genome editing of gene families and usually good candidate sgRNAs are tagged with low scores precisely because they match several locations in the genome, thus time-consuming manual evaluation of targets is required. Expand abstract.
14 days ago
9/10 relevant
bioRxiv

Comparison of CRISPR and marker based methods for the engineering of phage T7

Furthermore, we found the type II CRISPR-Cas system is easier to use and generally more efficient than a type I system in the engineering of phage T7. Expand abstract.
14 days ago
9/10 relevant
bioRxiv

Dissecting the roles of GRK2 and GRK3 in μ-opioid receptor internalization and β-arrestin2 recruitment using CRISPR/Cas9-edited HEK293 cells

By CRISPR/Cas9 we established HEK293 cells with knock-out of GRK2, GRK3 or both to dissect their individual contributions in {beta}-arrestin2 recruitment and -OR internalization upon stimulation with four different agonists. Expand abstract.
18 days ago
9/10 relevant
bioRxiv

CRISPR-based bioengineering of the Transferrin Receptor revealed a role for Rab7 in the biosynthetic secretory pathway

Here, we combined the retention using selective hook (RUSH) system to a CRISPR/Cas9 gene editing approach (eRUSH) to identify molecular players involved in the trafficking of neosynthesized Transferrin Receptor (TfR) en route to the plasma membrane (PM). Expand abstract.
22 days ago
9/10 relevant
bioRxiv