Found 242 results, showing the newest relevant preprints. Sort by relevancy only.Update me on new preprints

Temporal analyses of CRISPR-directed gene editing on NRF2, a clinically relevant human gene involved in chemoresistance

We reveal an initial snapshot of the schedule and processing of CRISPR/Cas in a lung cancer cell; information that will be useful as cell-based protocols are designed and advanced. Expand abstract.
4 days ago
9/10 relevant
bioRxiv

Site-Specific Generation of Protein-Protein Conjugates Using Native Amino Acids

The conjugation of cell penetrating peptides to CRISPR-Cas9 was shown to increase cellular genome editing efficiency by 20-fold relative to unmodified Cas9. Expand abstract.
5 days ago
4/10 relevant
chemRxiv

Potent CRISPR-Cas9 inhibitors from Staphylococcus genomes

Anti-CRISPRs (Acrs) are small proteins that inhibit the RNA-guided DNA targeting activity of CRISPR-Cas enzymes. Expand abstract.
6 days ago
9/10 relevant
bioRxiv

Repurposing the Streptococcus mutans CRISPR-Cas9 System to Understand Essential Gene Function

The CRISPRi tool and sgRNA library are valuable resources for characterizing essential genes in S. mutans, some of which could prove to be promising therapeutic targets. Expand abstract.
12 days ago
9/10 relevant
bioRxiv

High-throughput and all-solution phase African Swine Fever Virus (ASFV) detection using CRISPR-Cas12a and fluorescence based point-of-care system

We combine this powerful CRISPR-Cas assay with fluorescence-based point-of-care (POC) system we developed for rapid and accurate virus detection. Expand abstract.
13 days ago
9/10 relevant
bioRxiv

Structure and mechanism of a Type III CRISPR defence DNA nuclease activated by cyclic oligoadenylate

Can1 has a unique monomeric structure with two CRISPR associated Rossman fold (CARF) CARF domains and two DNA nuclease-like domains. Expand abstract.
18 days ago
9/10 relevant
bioRxiv

A nucleus-forming jumbophage evades CRISPR-Cas DNA targeting but is vulnerable to type III RNA-based immunity

These findings explain why many bacteria harbour both RNA- and DNA-targeting CRISPR-Cas systems. Expand abstract.
19 days ago
9/10 relevant
bioRxiv

A viral ring nuclease anti-CRISPR subverts type III CRISPR immunity

The AcrIII-1 family has a broad host range as it targets cA4 signalling molecules rather than specific CRISPR effector proteins. Expand abstract.
20 days ago
9/10 relevant
bioRxiv

Type IV CRISPR-Cas systems are highly diverse and involved in competition between plasmids

Furthermore, we find evidence for cross-talk between certain type IV and type I CRISPR-Cas systems that co-exist intracellularly, thus providing an answer to the enigmatic absence of adaptation modules in these systems. Expand abstract.
21 days ago
9/10 relevant
bioRxiv

CRISPR-Cas9 generated Pompe knock-in murine model exhibits early-onset cardiomyopathy and impaired skeletal muscle function

We therefore designed a CRISPR-Cas9 knock-in system targeting the Gaa gene to introduce the known pathogenic CRIM negative Gaa mutation c.1826insA (p. Expand abstract.
21 days ago
9/10 relevant
bioRxiv